Genetic Damage Caused by ALU Repeats in Breast Cancer
Abstract
We have developed a series of allele-specific PCR amplification procedures that allow us to amplify the flanking sequences from the most recent subfamilies of Alu elements in the human genome. There are approximately 1000 elements amplified in these experiments, and we have developed several strategies for amplifying specific subsets of these elements. The goal is to identify subsets of elements that can be amplified and displayed on a gel-based or subtractive method that will allow us to detect differences in these recent elements in breast tumor vs. normal tissue from a patient. This will allow us to detect either insertion of a new Alu element and assessment of the rate of gene damage from retrotransposition, as well as detect major sequence losses that encompass one of these elements.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2001
- Accession Number
- ADA398210
Entities
People
- Prescott L. Deininger
Organizations
- Tulane University of Louisiana