Cell Cycle Regulation by TGFb Signaling in C. elegans
Abstract
One of the most potent inhibitors of epithelial growth is TGF beta. Understanding the connection between TGF beta and cell cycle control is an important avenue of experimentation towards treating breast cancers. We are utilizing two complementary approaches in C elegans to find cell cycle regulatory genes that respond to TGF beta. First, we are using a cell cycle reporter, ribonucleotide reductase, to monitor cell cycle activation in mutagenized animals that are arrested at the dauer stage (a TGF beta induced developmental stage). Appropriate genetic strains have been constructed and tested, which will allow screening to proceed shortly. In a second approach, we are probing DNA microarrays with mRNAs collected from animals entering dauer. This experiment should identify genes whose regulation is altered by TGF beta as the animals undergo cell cycle arrest. During the next year, we hope to have isolated mutants from our screen and to have begun the molecular characterization of them.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2001
- Accession Number
- ADA398212
Entities
People
- Richard W. Padgett
Organizations
- Rutgers University–New Brunswick