Membrane-Bound Hyaluronidase in Breast Cancer Progression
Abstract
Malignant tumor cells utilize the abnormally expressed enzymes to digest the surrounding matrix and make their way to invade and metastasize. We hypothesize that membrane-bound hyaluronidase (HAase, PH-20) may be one of these enzymes that can promote the tumor progression. In first two years, we have demonstrated: (1) HAase stimulated the colony formation via release the FGF-2 from immobilized form to free form, and then effective in a paracrine manner. This is also true using gene transfection approach; (2) transfection HAase cDNA into MDA23l breast cancer cells could enhance the tumor growth in CAM system; (3) HAase transfected MDA23l cells formed aggressive tumor via enhancement of angiogenesis. In past year, we focused on the expression of membrane-bound hyaluronidase in human breast cancer tissues. We had carefully designed the peptides that represented the antigenic domain of this enzyme and prepared the antibodies. The immunohistochemic staining was performed in 57 cases of human breast cancer tissues. The data indicated that the antibody could stain tumor cells with little background in the stroma. The positive staining rate was 83.3% in metastatic breast cancer, while it was 55.5 in the non-metastatic tumors. Whether this enzyme can be a tumor marker will be further investigated.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2001
- Accession Number
- ADA398227
Entities
People
- Lurong Zhang
Organizations
- Georgetown University