Determination of Catechol Estrogen Adducts by High-Performance Liquid Chromatography: Establishing Biomarkers for the Early Detection of Breast Cancer
Abstract
In order to better understand the role of estrogen metabolism as it relates to breast cancer etiology, a new analytical technique that can measure CE and CE-DNA adducts at low endogenous levels is being developed. This new technique is based on HPLC analysis of fluorescent probes specific for CE and CE-DNA adducts. An extraction procedure employing a solvent mixture of chloroform, dimethylforamide, and acetic acid was developed to extract CE-DNA adducts, CE, and MPEM from rat breast tissue. This extraction procedure involves homogenization of the tissue in 20 ml of the solvent mixture, removal of the solvent, followed by HPLC analysis. Reaction of alpha,alpha-dibromomalonates occurs quickly with catechols, and this malonate system is being developed to produce fluorescent probes for HPLC analysis. Reaction of diethyl alpha,alpha-dibromomalonate with catechol results in ketal formation. The resulting ethyl ester is tranesterified to incorporate anthracene groups. A one-step process using alpha,alpha-dibromomalonamides is also under development. The aim of this work is to develop new biomarkers for the early detection of breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2001
- Accession Number
- ADA398228
Entities
People
- Douglas Stack
Organizations
- University of Nebraska Omaha