Biochemically Vulnerable Sites for Antifungal Intercession in the Control of Fungal Growth

Abstract

In the present grant period DNA array experiments were done in an approach towards identifying target genes that might be transcriptionally regulated by UPC2. Nylon membrane supported, genomic scale arrays were hybridized with radiolabeled cDNA from various strains, which were wild type or mutant with various alleles of UPC2. PDA1 was chosen to normalize the data. While many candidate genes were identified, expression of the genes ERG2, ERG2, ERG11, and ERG25 were selected for additional study. No difference from wild-type levels of ERG2 and ERG3 was apparent except in the upc2-1 mutant.

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Document Details

Document Type
Technical Report
Publication Date
Aug 31, 2001
Accession Number
ADA398294

Entities

People

  • Leo Parks

Organizations

  • North Carolina State University

Tags

Communities of Interest

  • Energy and Power Technologies

DTIC Thesaurus Topics

  • Abstracts
  • Anabolism
  • Anatomy
  • Antifungal Agents
  • Biological Sciences
  • Classification
  • Detection
  • Dna Microarrays
  • Fungi
  • Genes
  • Information Operations
  • Membranes
  • Military Research
  • Mutations
  • North Carolina
  • Pharmacologic Actions
  • Security

Fields of Study

  • Biology

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