Recombinant Polyclonal Antibody Libraries for Breast Cancer Therapy
Abstract
This project proposed the development of a recombinant polyclonal antibody library specific for the human breast carcinoma cell line BT-20. The proposed library would target multiple epitopes on the human cell line and also provide the genes necessary for the unlimited perpetuation of the defined antibody mixture. Ultimately, such antibody libraries could be used for treatment and diagnosis of breast cancer in humans. In the present study, an Fab phage display library specific for the BT-20 cells has been generated. To obtain a polyclonal antibody library of whole IgG antibodies that would mediate effector functions, a vector system for mammalian expression has been constructed. The new system uses restriction enzyme-based transfer of variable region gene pairs from a phage display to a mammalian expression vector. Improved methods for handling antigen-selected Fab phage display library were also developed. Construction of a new anti-BT- 20 library, using the improved vectors and methods, has begun.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2001
- Accession Number
- ADA398304
Entities
People
- Jacqueline Sharon
Organizations
- Boston University