Molecular Basis of Prostate-Specific Androgen-Independent Expression of a Homeobox Gene
Abstract
The goal of the work supported by this grant is to characterize the molecular basis of prostate specificity and androgen independence of the hoxb-13 gene with a view towards developing new treatments for advanced prostate cancer. To date, we have succeeded in generating a physical map of both the mouse and human hoxb-13 loci, and have sublconed regions both upstream and downstream of the coding region of both genes. Reporter gene constructs carrying up to 12.5 kb of the human hoxb-13 locus have been completed and transgenic mice carrying these constructs have been generated. Analyses of reporter gene expression in these mice have revealed that this region contains elements capable of directing a pattern of gene expression that recapitulates features of hoxb-13 expression but does not direct expression in the prostate gland. Transgenes carrying regions farther upstream as well as downstream of hoxb-13 loci are being constructed to widen the search for cis-acting elements that function in the prostate.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2001
- Accession Number
- ADA398350
Entities
People
- Charles J Bieberich
Organizations
- University of Maryland, Baltimore