Mapping Genetic Modifiers of Mammary Tumor Susceptibility
Abstract
Mutations in APC/Apc predispose both humans and mice to multiple polyps of the colon and small intestine. Min/+ mice carry a mutation at Apc and are predisposed to developing spontaneous intestinal and mammary tumors. On a C57BL/6J (B6) background, Min/+ mice develop spontaneous mammary tumors at a 5% rate. When injected with ENU (ethylnitrosourea), over 80% of B6 Min/+ female mice develop mammary tumors. l29/SvJ x B6 F1 mice that carry the Min mutation are resistant to mammary tumor development after ENU treatment. We produced backcross mice in order to map the modifier loci causing resistance in the (l29/SvJ x B6) mice. Preliminary analysis of SSLP markers spaced every 20-25 cM throughout the genome indicates the possibility of two regions that contain modifiers. One region maps to chromosome 6 near the marker D6Mit36. A congenic line, ROSA26, carrying this region on chromosome 6 from 129 on a E6 background had already been established. ROSA26 mice have a LacZ insertion flanked by 129 DNA in the modifier region on chromosome 6. ROSA26 x B6 Fl Min/+ mice are resistant to mammary tumors following ENU treatment. The ROSA26 mice will be used to study the biological effects of the potential modifier.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2001
- Accession Number
- ADA398591
Entities
People
- Amy Moser
- Rebecca L. Williams
Organizations
- University of Wisconsin–Madison