ErbB2 Regulation of c-src Activity in Breast Cancer

Abstract

Overexpression of ErbB2 in mammary epithelial cells increases activity, but not expression, of c-src. This effect is not mimicked by other transforming oncogenes. These results suggest that previous results in transgenic animals are likely to be due to direct effects on mammary epithelium, not to alterations in systemic physiology (such as hormone levels) or to altered epithelial stromal interactions. Results indicate that induction of c-src activity is mediated by increased activity of a src-directed phosphatase that removes an inhibitory phosphate at Y527. SHP2 appears to be a leading candidate for the phosphatase. In addition, the activation of c-src by ErbB2 appears to play a critical role in inducing a tumor phenotype. These results that strategies to modify src activity or the activity of the src-directed phosphatase(s) may prove useful in modifying tumor progression.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2001
Accession Number
ADA398670

Entities

People

  • Lewis G. Sheffield

Organizations

  • University of Wisconsin–Madison

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Acetic Acid
  • Acids
  • Breast Cancer
  • Cell Line
  • Cells
  • Chemistry
  • Culture Media
  • Cyanides
  • Enzymes
  • Epithelial Cells
  • Medical Personnel
  • Neoplasms
  • Peptides
  • Proteins
  • Regulations
  • Tyrosine

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular Biology and Genetics