Concurrent and Independent Genetic Alterations in Epithelial and Stromal Components of Breast Neoplasms: Implication for Tumor Development and Progression
Abstract
Our previous study on paraffin embedded tissues from patients with mammary carcinomas revealed a high frequency of loss of heterozygosity (LOH) in microdissected epithelial (EP) and adjacent or distant stromal (ST) cells. This study attempts to confirm previous findings on a larger scale and in a wider spectrum. Immunohistochemical and genetic analyses were performed on malignant and benign EP lesions with and without distinct ST alterations. Our findings show that morphologically comparable ST cells associated with malignant and benign lesions display a substantially different immunostaining patterns with antibodies to cell proliferation associated proteins, blood vessel components, and extracellular matrix molecules, and also show different frequencies and patterns of LOH at multiple chromosomal loci. ST cells from neither malignant nor benign lesions, however, display LOB or microsatellite instability with multiple DNA markers at chromosome l7p. These results are consistent with those of our previous studies, suggesting that 1 morphologically comparable ST cells in malignant and benign lesions are biofunctionally and genetically different and their genetic alterations correlate with those in their EP counterparts; 2 the biofunctions of ST cells may be independent of p53 gene regulation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2001
- Accession Number
- ADA398673
Entities
People
- Fatteneh A. Tavassoli
- Yan-gao Man