Shotgun Sequencing of Plasmids from Marine Sediment Bacteria - Genetic Exploration

Abstract

We have generated DNA libraries from 10+ plasmids (some samples contained more than one plasmid) and have performed enough DNA sequencing reactions on these estimated to yield approximately 5x sequence coverage. Sequence assemblies were generated for each of these and gap closure performed so far on five plasmids. These five have been analyzed using TIGR's automated bioinformatics tools. All the results are available internally and to our collaborators through a web based system.

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Document Details

Document Type
Technical Report
Publication Date
Sep 30, 2001
Accession Number
ADA398735

Entities

People

  • Jonathan A. Eisen

Organizations

  • J. Craig Venter Institute

Tags

DTIC Thesaurus Topics

  • Assembly
  • Bacteria
  • Biology
  • Biomedical Information Systems
  • Computational Biology
  • Computer Programs
  • Department Of Defense
  • Dna Sequence Analysis
  • Environment
  • Genetic Code
  • Genetic Structures
  • Genome
  • Information Operations
  • Microbial Genome
  • Sediments
  • Sequences

Fields of Study

  • Biology

Readers

  • Coastal Oceanography
  • Computational Modeling and Simulation
  • Molecular Genetics

Technology Areas

  • Biotechnology