Nitric Oxide in Mammary Tumor Progression

Abstract

Nitric Oxide (NO) is a potent bioactive molecule produced in the presence of endothelial (e), neuronal (n) and inducible (i) types of NO synthase (NOS) enzymes. We discovered that IL-2 therapy-induced capillary leakage was due to iNOS induction leading to overproduction of NO and its derivative peroxynitrite. We also found that tumor-derived NO promoted mammary tumor progression in nuce. eNOS expression by tumor cells was positively correlated with metastasis in spontaneous C3R/HeJ mammary tumors and transplants of two clonal derivatives of a spontaneous tumor differing in metastatic phenotype: highly metastatic C3L5 and weakly metastatic ClO cell lines. These cell lines also exhibited a parallel difference in invasiveness in vitro and growth rates as well as angiogenic abilities in vivo. A causal relationship between NO production by the tumor cells and invasive, migratory and angiogenic abilities was demonstrated.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2001
Accession Number
ADA398946

Entities

People

  • Peeyush K. Lala

Organizations

  • Western University

Tags

DTIC Thesaurus Topics

  • Cardiovascular System
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Lymphocytes
  • Medical Personnel
  • Organic Chemistry

Fields of Study

  • Biology

Readers

  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology