Tumor Targeting With Phage Library

Abstract

I have proposed to identify peptides that bind to the vasculature of prostate cancers by using a technique developed in our laboratory called 'in vivo phage display'. These peptides would have the potential to be used in drug targeting. The tumor-specific peptides will then be used to isolate their vasculature-specific receptors. The receptors could be specific markers for the vasculature of prostate cancers and lead to a greater understanding of what kinds of vascular surface proteins are up-regulated during prostate carcinogenesis. Because of the difficulty in the experimental procedure, I took an alternative approach, mRNA profiling by GeneChip or cDNA microarray. To this end, I have accomplished the following goals: (1) Established GeneChip and cDNA Microarray systems in our laboratory. (2) Established an in vitro transcription method to amplify limiting amount of RNA that can be obtained from mice. (3) Improved endothelial cell purification procedures by adopting magnetic beads to increase yield. (4) Screened endothelial cell specific markers from lung and kidney.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2001
Accession Number
ADA399090

Entities

People

  • Jih-tung Pai

Organizations

  • Sanford Burnham Prebys Medical Discovery Institute

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Biomedical Research
  • Buildings And Structures
  • Cell Line
  • Cells
  • Data Sets
  • Dna Microarrays
  • Electronic Mail
  • Endothelial Cells
  • Information Operations
  • Neoplasms
  • Prostate
  • Prostate Cancer
  • Research Facilities
  • Targeting

Fields of Study

  • Biology
  • Chemistry

Readers

  • Molecular Genetics
  • Oncology (Cancer Research).