Luminescent Nanoparticles for High Sensitivity Biosensing
Abstract
Water-soluble luminescent nanoparticles have been fabricated with suitable surface chemistry to attach biomolecules, engineered to attach via spontaneous self-assembly. The resulting bio-conjugates have been characterized and the effects of pH and ionic strength on stability examined. Additionally, methods to improve bacterial expression and purification of the fusion proteins have been developed. QDs coated with protein G, which binds antibody, have been utilized in developing sensitive immunoassays. Finally, viral capsids, which are made of repeating subunits, are being utilized as signal amplifiers, enhancing the sensitivity of optical sensors with the goal of detecting single molecular events.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 31, 2000
- Accession Number
- ADA399563
Entities
People
- G. Anderson
- H. Mattoussi
- M. Mauro
- R. Banahalli
Organizations
- United States Naval Research Laboratory