Rapid Screen for Tumor Suppressor Genes on Chromosome 19p13
Abstract
We have previously reported that a region on chromosome 19p 13 displays extremely high rates of loss of heterozygozity (LOH). LOH is a hallmark for the existence of tumor suppressor gene. We proposed to identify potential candidates through the use of cDNA microarrays. Therefore we would spot cDNAs from genes mapping to the high LOH area onto chips, and hybridize them with genomic DNA from breast tumors. The same arrays could be used in a subsequent study to measure RNA expression of genes from the high LOH area. In studies performed outside this proposal, we would analyze potential candidates through functional studies. Using available sequence information we have generated a list of 120 cDNAs mapping to the high LOH region as well as genes known to be lost or amplified in breast cancer. We have obtained the appropriate IMAGE clones, and PCR amplified the genes. Despite considerate problems with those clones we have now finished generating the correct cDNA pool, and we are planning on printing the slides in the near future.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2001
- Accession Number
- ADA400057
Entities
People
- Steffi Oesterreich
Organizations
- Baylor College of Medicine