Molecular Characterization of Resistance

Abstract

This is an IDEA Award in which we propose to characterize the molecular events driving acquired antiestrogen resistance. We have begun to acquire extensive gene expression microarray data on a panel of eight different antiestrogen resistant cell lines and their parental cells. The analysis of these data is ongoing and the study is on-track. Several new algorithms for data analysis are underdevelopment. One of these algorithms, a new method for data normalization, has been developed and tested and a manuscript is now in press. Our data with NFkB and IRF-I suggest we may be on the right track to identifying new signal transduction pathways associated with acquired antiestrogen resistance. For example, these data show that resistant cells are more sensitive to inhibition of NFkB. Overexpression of IRF-1, which is suppressed by estrogens and induced by antiestrogens is associated with reduced cell proliferation.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2001
Accession Number
ADA400058

Entities

People

  • Robert R. Clarke

Organizations

  • Georgetown University

Tags

DTIC Thesaurus Topics

  • Algorithms
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Culture Techniques
  • Data Analysis
  • Data Sets
  • Estrogens
  • Gene Expression
  • Genes
  • Genetics
  • Mammary Glands
  • Neural Networks
  • Pilot Studies
  • Proteins
  • Resistance

Readers

  • Breast cancer cell signaling and growth regulation.
  • Clinical Trial Research.
  • Distributed Systems and Data Platform Development