Procathepsin D Stimulation of Human Breast Cancer Cell Growth
Abstract
Nine different clones transfected with human pCD cDNA and eight clones transfected with cDNA with deleted APpCD have been established and tested. Our results showed that the individual clones differ in secretion of pCD. These clones not only secrete pCD into the medium, but can also react to the estradiol stimulus by proliferation. Similar results have been obtained when the tumor growth has been evaluated in vivo and in vitro. Matrigel assay demonstrated that the transfection with pCD with deleted activation peptide did not influence the invasiveness of the clone. These data demonstrate that the proliferation and invasiveness of breast cancer cells closely correlates with production and secretion of pCD and the presence of the APpCD. After establishing that the fragment 27-44 is the part responsible for the pCD binding to cells, we focused our attention on this particular fragment. We found that the biological activity can be located into the fragment AA 36-44. The cancer cells reacted to the addition of AA 36-44 fragments with the same level of proliferation as to the addition of pCD. When this fragment was used for inhibition of the pCD-FITC binding to the cancer cells, this fragment was comparable to the unlabeled pCD. We prepared a library of synthetic peptides with a single amino acid substitution based on the results mentioned above.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2001
- Accession Number
- ADA400390
Entities
People
- Vaclav Vetvicka
Organizations
- University of Louisville