Enzymatic Degradation of HD
Abstract
An enzyme, derived from a Rhodococcus bacterium, was found to have catalytic activity with HD as the substrate. The enzyme has previously been characterized as a hydrolytic dehalogenase and apparently catalyzes the hydrolysis reaction of HD, because chloride is released stoichiometically. The enzyme is precipitated between 60 and 100% ammonium sulfate saturation, binds to Q Sepharose and elutes at an ammonium sulfate concentration of about 0.1%. The enzyme exhibits saturation kinetics with HD as its substrate. The Km is about 15 mM HD and the V(sub max) occurs at about 25 mM. It exhibits fairly conventional temperature and pH effects with a temperature optimum of about 35 deg C and a pH optimum of about 7.0.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2001
- Accession Number
- ADA400437
Entities
People
- Steven P. Harvey
Organizations
- Edgewood Chemical Biological Center