Genetic and Dynamic Analyses of Murine Peak Bone Density
Abstract
A comprehensive genetic assessment was undertaken for Quantitative Trait Loci (QTLs) that regulate acquisition of peak bone density using the low density C57BL/6J (B6) and the high density CAST/EiJ (CAST) and C3H/HeJ (C3H) inbred strains. Genome wide analyses of (B6 x CAST)F2 and (B6 x C3H)F2 progeny yielded 8 QTLs in the B6CAST-F2 F2 and 10 QTLs in the B6C3H-F2 progenies, respectively. Heritability of BMD ranged from 57 to 87%, demonstrating the strong genetic basis for this trait. Locations of the QTLs (LOD scores > 2.9) were established, major effects and modes of inheritance were established, and B6 background congenic strains were established to pursue biological and fine mapping/cloning of genes. Genes common to femurs and to vertebrae were found, in addition to genes unique to each bone site. Biological studies of the progenitor B6, C3H, and CAST inbred strains reveal that there are both formation and resorption processes actively in place that contribute to the differences in bone mineral density, microstructure and strength differences in these strains. Studies of QTL gene locations, structure, biomechanical, microarchitectural, and process of bone turn-over are currently being pursued as well as being planned for the future.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2001
- Accession Number
- ADA400443
Entities
People
- Wesley G. Beamer