Tropomyosin-1: A Putative Tumor-Suppressor and a Biomarker of Human Breast Cancer
Abstract
Changes in the expression of microfilament-associated proteins such as tropomyosins, are associated with the transformed phenotype. In this work we have tested the hypothesis that TM1 is putative biomarker and tumor suppressor of breast cancer. Using TM1-specific antibodies RNA probes, we are assessing the expression of TM1 in the primary breast tumor specimens. Restoration of TM1 expression in MCF-7 (MCF-7/T) cells resulted in slower growth rate. MCF-7/T cells remain sensitive to growth control by estrogen, and TM1 re-expression appears to strengthen the interaction of E-cadherin-catenin complex with microfilaments. More significantly, MCF-7/T cells failed to grow under anchorage-independent conditions. Thus, TM1 appears to be essential for normal growth and differentiation of mammary epithelium. Abolition of TM1 expression appears to be necessary for malignant transformation by multiple oncogenic modalities. Together, our findings demonstrate that TM1 is a class II tumor suppressor.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2001
- Accession Number
- ADA400464
Entities
People
- Gaddamanugu L. Prasad
Organizations
- Wake Forest University