NF-1 - Regulated Adenylyl Cyclase/c AMP Pathways
Abstract
This project takes Drosophila NF1 mutants and mouse Nf1 mutants as models to investigate NF1-dependent regulation of the cAMP pathway. The study is intended to expand the knowledge of the genes that contribute to NF beyond the GAP-related domain in NF1. In the last year, our work has been mainly focused on two aspects. First, the effort has been devoted to determine whether AC activity can be regulated by NF1 in vertebrates. Second, we investigated whether Ras can regulate AC activity in Drosophila. These studies have led to findings that expression of the human NF1 gene was capable of rescuing AC defects in Drosophila and G protein stimulated AC activity is reduced in mouse Nf1 knockout It was also revealed that Ras, but not Ras-like small G proteins, was able to stimulate AC activity in a NF1 dependent manner. We are continuing to pursue a molecular understanding of this regulation and whether this pathway contributes to pathogenesis of NF1.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2001
- Accession Number
- ADA400477
Entities
People
- Yi Zhong
Organizations
- Cold Spring Harbor Laboratory