Molecular Detection of Circulating Cancer Cells for Early Diagnosis of Breast Cancer
Abstract
Goal: The goal of this project is to detect circulating cancer cells using breast-specific tumor markers in the blood from 200 patients who have suspicious mammograms and breast tissue biopsies. These molecular detection results will be correlated with the biopsy results. Patient accrual: Because this study just recently opened in July, 2001, we have collected blood from 54 patients with suspicious mammograms immediately prior to a breast tissue biopsy. These patients were informed of the investigational aspects of this study and have given written consent in accordance with institutional and federal guidelines. Molecular detection progress: Isolation of mononuclear cells by density gradient centrifugation and immunomagnetic capture of epithelial cells from all the collected blood samples have been performed. Isolation and reverse transcription of mRNA from each accrued sample are currently in progress. After reverse transcription, cDNA from each sample will be amplified using fluorescent-based kinetic PCR with gene-specific primers. Circulating cancer cells will be detected using mammaglobin, B305D, and B726P, which are breast-specific genes, and gamma-aminobutyrate type A receptor it subunit, cytokeratin-1 9, and beta-actin. Summary: We have collected 25% of the proposed 200 sample accrual, and the molecular detection of circulating cancer cells from the collected samples is in progress.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2001
- Accession Number
- ADA400500
Entities
People
- Monica M. Reinholz
- Patrick C. Roche
Organizations
- Mayo Clinic