Evaluation of a Mouse Model for Prophylactic Mastectomy in Mice Genetically Predisposed to Breast Cancer
Abstract
Specific Aim I: To evaluate the role of mammary cell volume reduction in inducing morphologic changes in residual tissue. Muller (9) observed that the mammary tissue in these mice is initially hyperplastic and eventually becomes cancerous. Therefore any hyperplastic tissue remaining after mastectomy is at risk for developing breast cancer. In these experiments, we assessed the extent of that risk by evaluating the volume and morphology of mammary tissue remaining after PM. We evaluated the volume of residual mammary tissue after mastectomy by using autopsy studies performed one week after PM . This time interval was chosen to allow full recovery from surgery and it precedes the development of scar tissue that would interfere with pathological evaluation. The skin was cut in .25 inch serial sections and stained with hematoxlyn and eosin. The specimens will then be examined for the presence of mammary tissue. This required approximately 20 animals. Note; the original proposal called for the use of anti-MMTV antibody to screen for the presence of residual mammary gland tissue. Unfortunately, the quantity of antibody obtained was too small to screen a large number of mice. As a result only H&E staining was used for these experiments.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2001
- Accession Number
- ADA400613
Entities
People
- Yvedt L. Matory
Organizations
- Brigham and Women's Hospital