Molecular Mechanism for Loss of Cell Adhesion in HER-2/neu Overexpressing Tumor Cells
Abstract
The HER-2/neu proto-oncogene is amplified and overexpressed in approximately 25% of breast cancers. Amplification and overexpression of HER-2/neu is correlated with poor patient prognosis, lack of responsiveness to tamoxifen treatment, responsiveness to adriamycin chemotherapy and responsiveness to Herceptin anti-HER-2/neu immunotherapy. In this proposal we are characterizing changes in cell adhesion related to HER-2/neu overexpression. We have engineered two human cell lines to overexpress HER-2/neu Relative to parental control cell lines HER-2/neu overexpressing cell lines showed a loss of cell adhesion on selected extracellular matrix proteins, especially denatured collagen type I and vitronectin. The findings suggest that the alphavbeta3 integrin receptor may he involved in this process since alphavbeta3 integrin receptor mediates binding to these extracellular matrix proteins.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2001
- Accession Number
- ADA400616
Entities
People
- Michael F. Press
Organizations
- University of Southern California