Nanoscale Field Localization for Manipulation and Probing of Computationally Interesting Biomolecules
Abstract
Several new and important results were enabled by this grant, which was to build a bioelectronic interface to single protein molecules. This interface consists of both environmental and mechanical supports for a membrane protein. While supported membranes are being studied in many laboratories, single channel probing has been performed almost exclusively with voltage-clamp techniques, by using fragile glass pipettes, or with cumbersome planar bilayer equipment unsuitable for field deployment. Single channel recording cannot yet be done on supported membranes. In this work, multifunctional scanned probe microscope tips were designed and fabricated to use in accessing the state of a membrane porin protein using microwave reflection and transmission, as well as fluorescent probing using photodiode probes. Specific accomplishments were: development of a micromachined probe process and several probes to perform combined scanning force microscopy together with microwave probing, development of a companion process for combined optical and scanning force probing, imaging of calcium waves in brain slices, and preliminary results on single-protein probing with both direct-current and high-frequency probes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2001
- Accession Number
- ADA400783
Entities
People
- D. W. Weide
Organizations
- University of Wisconsin–Madison