Fluid Flow Sensitivity of Bone Cells as a Function of Age

Abstract

The third year of the proposal has gone quite well. Aims 1 and 2 are essentially complete. Major findings include: (1) Confirmation that GJIC contributes to bone cell PGE2 but not Ca(2+) response to fluid flow. This was demonstrated in two different osteoblastic cell lines using several different techniques to inhibit GJIC. (2) Confirmation that ROS cells do not display an increase in Ca(2+) in response to fluid flow. (3) Discovery that the lack of a Ca(2+) response to fluid flow in ROS may be due to lack of purinergic P2Y2 receptors. (4) Discovery that an ATP interaction with purinergic receptors contributes to fluid flow responsiveness in both ROS and MC3T3-E1 cells. (5) Discovery that there is a refractory period for fluid flow induced calcium mobilization in osteoblastic cells. The last year of the project will focus on completion of aim 3.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2001
Accession Number
ADA401057

Entities

People

  • Henry J. Donahue

Organizations

  • Pennsylvania State University

Tags

DTIC Thesaurus Topics

  • Age Groups
  • Biomedical Engineering
  • Bone And Bones
  • Bone Diseases
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Flow
  • Fluid Flow
  • Materials Testing
  • Medical Personnel
  • Orthopedics
  • Osteogenesis
  • Osteoporosis

Fields of Study

  • Biology

Readers

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