Markers of Increased Risk in Pre-Invasive Breast Cancer
Abstract
The risk of recurrence and progression of ductal carcinoma in-situ (DCIS) of the breast is best designated by morphological indicators including nuclear grade and presence of necrosis. To identify molecular alterations underlying these morphological features we have compared gene expression within a cohort of 10 cases of DCIS (6 high-grade or intermediate-grade DCIS with necrosis and 4 low-grade DCIS) using microdissection and cDNA microarray. A set of 42 cDNAs, from a group of 1,500, was identified that were consistently differentially expressed and whose expression profile clustered with DCIS grade. Amongst this set, the angio-associated migratory cell protein (AAMP) was identified as an mRNA that is consistently higher in high-grade DCIS (p = 0.0095) and found to be overexpressed in the T47D breast cancer cell line subjected to hypoxia. Differential expression was confirmed by quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and in situ hybridization analysis of 37 DCIS. AAMP mRNA tended to be associated with high and intermediate-grade DCIS and DCIS with necrosis. The present study shows that while levels of gene expression are mostly similar between morphologically different DCIS, consistent differences in expression of a subset of genes can be identified between low-grade and high-grade DCIS.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2002
- Accession Number
- ADA401098
Entities
People
- Adewale Adeyinka
- Peter H. Watson
Organizations
- University of Manitoba