The Single Prostate Cell Transcriptome as Biological Assay
Abstract
Technological advances in robotics and image analysis software have expanded the capabilities of large-scale strategies to provide for a more complete analysis of molecular differences between tissues or cells of interest. The research accomplished to date has demonstrated the ability to reproducibly isolate defined prostate cell populations by microdissection and flow cytometry. Gene expression studies of the cells purified by flow-cytometry reveal an altered expression profile that we believe results from the tissue dissociation/dispersion procedures. Ongoing and future work employs microdissection as the procedure of choice for specific cell-type analyses. The microdissection approach using a laser capture microscope is an efficient procedure for isolating cells representing abundant cell types, and we have isolated, purified, and analyzed the gene expression profiles from luminal epithelium and stromal elements. We have greatly expanded the database of sequences acquired from specific prostate cell types, and constructed arrays encompassing a wide range of diverse genes (n=6,OOO). In preliminary experiments we have used amplified cDNA probes isolated from small cell numbers to assess the gene expression profiles of defined cell types.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2002
- Accession Number
- ADA401685
Entities
People
- Peter S Nelson