Detection of Polyclic Aromatic Hydrocarbon Adducts Using Electrochemistry/Electrospray-Mass Spectrometry
Abstract
Electrochemistry (EC) and Electrospray Ionization (ESI) mass spectrometry were coupled online to detect the formation of adducts between polycyclic aromatic hydrocarbons (PAHs) and biological nucleophiles. A simple tubular electrode electrochemical cell was used to oxidize anthracene and benzoApyrene, and a mass spectrometer was used to detect their reaction products. An optimum electrolyte concentration was determined for detecting adducts of anthracene. A lower limit of detection for anthracene adducts was demonstrated for the EC/ESI technique over ESI alone. Several operating parameters were also investigated for optimal PAR adduct detection: the flow rate of the solution, the high voltage applied to the ESI needle, the voltage applied to the capillary, and the collection time in the hexapole.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2002
- Accession Number
- ADA401907
Entities
People
- David A. Pfahler
Organizations
- Air Force Institute of Technology