Patterning Micro- and Nanometer Scale Lipid Bilayers Using a Polymer-Based Lift-Off
Abstract
Lipids, antibodies, antigen, avidin and biotin have been patterned at nanoscale resolution for the precise immobilization and stimulation of immunological cells. We demonstrate that biomaterial can be patterned on silicon using a photolithographically patterned polymer lift-off technique. The nanoscale pattern is realized as the polymer is mechanically peeled away in one contiguous piece in solution. The 600 nm to 67 microns biomaterial patches provide a synthetic biological substrate for biochemical analysis. 100-nm unilamellar lipid vesicles spread to form a supported lipid bilayer on thermally oxidized silicon surface as confirmed by fluorescence recovery after photobleaching (FRAP) This method provides a new technique for immobilizing biomaterials, capturing antibodies from solution, and providing a platform for nanoscale antigenic stimulus.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2002
- Accession Number
- ADA402424
Entities
Organizations
- Cornell University School of Applied and Engineering Physics