Nanometer Scale Antibody Patterning for Directed Cell Immobilization and Stimulation
Abstract
Antibodies (AB) are patterned at nanoscale precision for the precise immobilization and stimulation of immune cells. We demonstrate that the antigen bovine serum albumin (BSA) can be patterned on silicon using a photolithographically patterned polymer lift-off technique. The nanoscale pattern is realized as the polymer is mechanically peeled away in one courageous piece in aqueous solution. Anti-BSA AB are bound specifically to BSA to create a pattern of oriented Ab that provides a Surface for eosinophils immobilization and degranulation. The patterns ranged from 0.36 micrometers(2) to 4,489 micrometers(2), appropriate dimensions for the 10-14 micrometers diameter eosinophils cells, This method provides a new technique for immobilizing cells onto nano- and micrometer scale patterns for analyzing cellular biochemical cascade events such as degranulation and studying cellular morphological changes in response to defined nanoscale antigenic stimulus.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 22, 2002
- Accession Number
- ADA402464
Entities
People
- Reid N. Orth
Organizations
- Cornell University