The Role of Galectin-3 in the Interactions Between Breast Carcinoma Cells and Elastin
Abstract
Our overall objective is to delineate the role of galectin-3 in the interactions between breast carcinoma cells and elastin. Therefore the scope of this proposal is to prove to ourselves and convince the scientific community that expression of galectin-3 by breast cancer cells is not only important for growth, but also the interaction of these cells with elastin, an interaction which has significant consequences when one considers the propagation of metastatic cells in elastin rich tissues. We have at this point demonstrated by transfecting tumorigenic breast epithelial cells with antisense oligos to galectin-3 that indeed galectin-3 is critical for the interaction of these cells elastin. We have also demonstrated that fetuin, a serum glycoprotein is a necessary for these tumor cells to release galectin-3 which is then used to ligate the cells to elastin. We have also narrowed the motif in elastin which is involved to the VGVAPG chemotactic domain. As for the galectin-3 the interacting domain encompasses the carbohydrate recognition domain. In the next year we hope to pinpoint the peptide domain(s) in galectin-3 which are responsible for galectin-3/elastin interaction.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2001
- Accession Number
- ADA403324
Entities
People
- Josiah Ochieng
Organizations
- Meharry Medical College