Prevention of Breast Cancer by Targeted Disruption of Breast Epithelial Cells
Abstract
We proposed to test the validity of the hypothesis that introduction of recombinant toxins into the confines of the mammary ductal tree through the teat will kill breast epithelial cells. To this end, we designed a new toxin to target epithelial cells. We have completed the construction of a chimeric toxin consisting of the protein transduction domain of the HIV TAT gene to target and enter the cells, and the VPR gene of HIV to cause apoptosis. Expression of this protein in bacteria, and its purification is in progress. In animal experiments, we also demonstrated the concept that both chemopreventive and chemotherapeutic drugs could be clinically effective when delivered by the intraductal route. This allows us to ask if, by this route of administration, systemic toxicity could be avoided. In the third year of this grant we will test the efficacy of this toxin in cultured human and rat breast cells. If effective, we will test the toxin in the rat model system.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2001
- Accession Number
- ADA403346
Entities
People
- Saraswati Sukumar
Organizations
- Johns Hopkins University