Regulation of BRCA-1 Gene Expression and Mammary Tumorigenesis by the Brn-3B POU Family Transcription Factor
Abstract
The Brn-3b POU family transcription factor is over-expressed in breast cancer cells compared to normal mammary cells. In this project, we have stably transfected the human breast cancer cell line MCF-7 with vectors expressing the sense or anti-sense strand of Brn-3b so as to isolate cell lines with respectively enhanced or reduced Brn-3b levels. The cell lines with enhanced Brn-3b levels show enhanced growth rate, saturation density, cell division, anchorage independence in vitro and enhanced ability to form tumors in vivo. In contrast, the cell lines with reduced Brn-3b show reductions in all those growth parameters. In addition, the cell lines with altered Brn-3b levels show altered expression of genes such as BRCA-1 and the estrogen receptor whose expression is known to be modulated in breast cancer. These findings thus establish Brn-3b as a key factor regulating gene expression and growth of human breast cancer cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2001
- Accession Number
- ADA403379
Entities
People
- David S. Latchman
Organizations
- University of London