Breast Tumor pH: Design, Evaluation, and Application of Novel Reporter Molecules
Abstract
Systemic drugs are one of the most potent means for controlling beast cancer. Their ability to kill cancer cells often depends on the presence of appropriate physiological conditions, e.g., the uptake and retention of 5-fluorouracil (5-FU) may be influenced by pH. This opens the possibility of predicting which tumors will show best response by measuring pH a priori or modulating tumor physiology to optimize tumor selectivity. We have developed a novel class of non-invasive NMR pH indicators and propose a novel second generation of enhanced indicators to investigate breast tumor pH. We have now successfully synthesized our first trifluoromethyl pyridoxol pH indicator, which displays a narrow 19 W NMR resonance and is sensitive to pH. This molecule has the advantage of three times the signal to noise of previous indicators. It has been tested in blood and will be evaluated in breast tumors in the near future. We will also investigate the feasibility of manipulating pH in order to enhance breast tumor uptake. An exciting additional finding is that the NMR approach is potentially suitable for assaying additional tumor characteristics and we have invented a novel concept in testing for gene transfection.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2001
- Accession Number
- ADA403387
Entities
People
- Ralph P. Mason
Organizations
- University of Texas at Dallas