Analysis of Metastasis in Transgenic Mice

Abstract

The goal of this project is to analyze the metastasis of mammary tumors in transgenic mice. This will make use of fluorescently labeled tumor cells to allow in vivo visualization of the tumor cells in the primary tumor as well as in target organs. For the first year of this project we have generated 3 transgenic lines carrying the MMTV-GFP expression construct. We have evaluated GFP fluorescence in one of these lines (founder 25) and are in the process of testing two others. Heterozygotes derived from founder 25 show GFP fluorescence in mammary ducts and are now being crossed with transgenic mice carrying the HER2/neu or polyoma middle T transgene.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2001
Accession Number
ADA403427

Entities

People

  • Jeffrey E. Segall

Organizations

  • Albert Einstein College of Medicine

Tags

DTIC Thesaurus Topics

  • Animals
  • Biomedical Research
  • Breast Cancer
  • Breeding
  • Cancer
  • Cell Movement
  • Cells
  • Demographic Cohorts
  • Fluorescence
  • Genetically Modified Organisms
  • Laboratory Animals
  • Mammary Glands
  • Materials
  • Metastasis
  • Microscopy
  • Neoplasms
  • Recombinant Dna

Fields of Study

  • Biology

Readers

  • Molecular and genetic basis of cancer.
  • Oncology (Cancer Research).