The Role of Mitochondria in the Detection of Infrared Light Sources by Mammalian Cells

Abstract

We have discovered a novel expression of the function of live mitochondria, which we called Reversible Excitation Light Induced Enhancement of Fluorescence (RELIEF)'. It appears restricted to mitochondria of mammalian cells. Virus infected cultured cells change their expression of RELIEF within hours of infection. Neurons in culture express RELIEF quite strongly and change the expression as they develop, age and degenerate. It offers a simple fluorescence assay that can be completed in minutes. It can be observed in live cells, in the extracellular medium, and in isolated mitochondria. The secretion of the fluorescent material into the extracellular medium led us to a search for the secretory channels. We found a class of new objects which we call I-centers' (water structuring centers) on the cell surface. At present it is not yet clear whether they related to the secretion channels of the RELIEF-related materials.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2002
Accession Number
ADA403584

Entities

People

  • Guenter Albrecht-buehler
  • Robert L. Rea

Organizations

  • Northwestern University

Tags

DTIC Thesaurus Topics

  • Biology
  • Cell Line
  • Cells
  • Culture Techniques
  • Cultured Cells
  • Curve Fitting
  • Detection
  • Electrical Engineering
  • Fungi
  • Infection
  • Light Sources
  • Materials
  • Medical Personnel
  • Mitochondria
  • Organic Materials
  • Universities
  • Wound Infections

Fields of Study

  • Biology

Readers

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  • Systems Analysis and Design