Role of Melatonin in the Prevention of Breast Cancer in Patients with Cystic Breast Disease
Abstract
Melatonin and breast cyst fluids (BCFs) both exert antiproliferative effects on breast cancer cells that may be mediated by growth factors. The primary objectives of this study are to establish a clinical BCF sample bank among patients with gross cystic breast disease to study of the relationship between this disease, melatonin and related growth agents in BCF, and breast cancer risk. BCF samples from participating patients will be used to elucidate the contribution of melatonin and related growth agents (EGF, TGF-beta, DHEA-S) to the oncostatic effects of BCFs on MCF-7 human breast cancer cells. Our progress to date includes the establishment of a BCF sample bank and continued patient enrollment in year 2. Assay procedures were established for each biochemical analyte specified in the statement of work and BCF samples were analyzed for: melatonin, sodium, potassium, TGF-beta 1, TGF-beta 2, EGF, and DHEA-S. Preliminary time course, dose-response, and repeatability experiments were initiated using BCF to treat MCF-7 cells. A protocol was established for exposing MCF-7 cells with BCF and treatments were performed using samples from recruited patients. In summary, cell culture experiments using BCF samples and corresponding biochemical composition assays continued in year 2 and will progress into year 3.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2001
- Accession Number
- ADA403645
Entities
People
- Robert Wells
Organizations
- Colorado State University