Rolling Circle Transcription of Ribozymes Targeted to ras and mdr-1
Abstract
We report progress on the use "DNA nanocircle vectors" to encode the synthesis of ribozymes in living cells. Ribozyme-encoding circular DNA randomized libraries were used to select for strongly transcribed sequences with specific RNA polymerases. We identified new ssDNA promoters for bacterial enzymes and built new DNA nanocircles encoding ribozymes. These were demonstrated to be biologically active in bacterial cells. A similar strategy may one day be useful in the future for inhibiting genes (such as breast cancer-associated genes) in human cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2001
- Accession Number
- ADA404587
Entities
People
- Eric T. Kool
Organizations
- Stanford University