A Breast Tumor Suppressor Gene on 8p22: Identification by the Genetic Suppressor Element Approach
Abstract
We are currently investigating a putative tumor suppressor gene (TSG) located at chromosomal band 8p22 that is involved in breast cancer. We have proposed to apply the genetic suppressor element (GSE) approach to the identification of this TSU. Briefly, a library of short gene fragments will be introduced into a cell line which demonstrates suppression of clonogenicity in soft agar with the transfer of chromosome 8. Presumably, the 8p22 TSU is responsible for the suppression of clonogenicity, and the introduction of an active" USE from the library into the suppressed cells should inhibit the 8p22 TSU contained in the hybrid cells and allow reversion back to the parental phenotype, the ability to grow in soft agar. Any clones that form will be isolated and further evaluated, as a candidate for the TSU. Currently, a complete list of ESTs have been identified in the 8p22 interval using various methods. A USE library consisting of these ESTs has been constructed and evaluation of the library is currently underway. All preliminary optimization experiments for retroviral library delivery have been completed. The majority of candidate ESTs have been partially characterized and full length cDNAs obtained. Additionally, one candidate has been identified as a likely potential downstream target of the 8p22 TSU from differential gene expression studies, this gene is currently being analyzed individually in our MB231 hybrid/parental assay system to evaluate its functional significance in breast cancer cells
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2001
- Accession Number
- ADA404593
Entities
People
- Kumarika Banerjee
Organizations
- University of Illinois at Chicago