99M Tc-peptides for Detection of Breast Cancer
Abstract
The goal of this project is to identify peptides from phage display peptide libraries which bind with high affinity to the mutant EGFRvIII receptor present in breast tumors. The peptides selected are radiolabeled with technetium-99m (99mmTc) and tested for their potential as possible agents in the detection of breast cancer. Using commercially available phage display peptide libraries, we have identified four consensus peptides that show affinity for cells expressing the mutant EGFRvIII receptor. Characterization of these selected peptides was by ELISA and radiolabeled cell binding studies. First, the labeled phage were tested in in wfro assays and in mice with tumors. Specific binding of the labeled phage to the study cells was found relative to the control cells. Also, mice with tumors expressing the mutant receptor showed enhanced accumulation of the labeled phage over mice with tumors expressing the wild-type receptor. The consensus peptides were identified through analysis of the phage DNA. The peptides were synthesized commercially, then conjugated to a chelator for radiolabeling with 99rnTc. All peptid9e%s1 have been tested in in %4tro assays and tested in tumor bearing mice. The in vivo studies show that the Tc-peptides clear the drculation quickly and demonstrate accumulation in breast tumor.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2001
- Accession Number
- ADA404606
Entities
People
- Mary Ruschowski
Organizations
- University of Massachusetts