Involvement of a Novel Rho GTPase Activating Protein in Breast Tumorigenesis
Abstract
This project is aimed at studying specific Rho signal transduction pathways in normal and breast carcinoma cell lines. The members of Rho family of Ras-related proteins are critical downstream components of signaling pathways. The Rho GTPases are involved in a variety of cellular functions. The Rho GTPases are active when bound to GTP and switch to inactive form when the protein bound GTP is hydrolyzed. Rho GTPase activating protein (Rho GAP) converts active Rho into inactive Rho. We have cloned a Rho GAP encoded by the human chromosome l3ql2. We have characterized the biochemical activity of the bacterially expressed Rho GAP by investigating its effect on native GTPase activity of Rho protein. The physiological role of Rho GAP was established by transfecting cells with Rho GAP construct and showing its effect on actin reorganization. Furthermore, constructs containing altered sequences of Rho GAP cDNAs were made to investigate their effect on cellular phenotypes of normal and breast carcinoma cell lines.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2001
- Accession Number
- ADA404607
Entities
People
- G. M. Nagaraja
- Rajendra P. Kandpal
Organizations
- Fordham University