Applications of a Novel Nucleic Acid Detection Method in Breast Cancer Analysis of Overexpression of HER-2/neu and TAK
Abstract
The proposal "Applications of a Novel Nucleic Acid Detection Method in Breast Cancer: Analysis of Overexpression of HER-2/neu and FAK" is aimed at utilizing new biosensors based on guanine electron transfer to quantitate messenger RNA for breast cancer genes. The biosensors are based on a scheme involving abstraction of electrons from the guanines of immobilized RNA to generate a signature current for a specific gene. The purpose of the proposed research is to demonstrate that the guanine electron transfer technology can be used to detect overexpressed RNAs in real biological samples. In the first year, electrochemical detection of specific genes using Ru(bpy)3(2+)-mediated electrochemistry was demonstrated, In the second year, fragment size was determined using electrochemistry, which may enable new methods for RFLP analysis or detecting microsatellites. This year, novel molten salts of DNA were prepared that can be immobilized on small electrodes for ultra-miniaturized detection. These materials have allowed for electrochemical detection of DNA on microlocations as small as 5 microns; present DNA chips are generally limited to microlocations in the 100 micron range.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2001
- Accession Number
- ADA404625
Entities
People
- Herbert Thorp
Organizations
- University of North Carolina at Chapel Hill