A Novel RNA Virus System for Selective Killing of Breast Cancer Cells
Abstract
The goal of our work is to develop novel methods based on rSV5 for killing tumor cells. We have made significant progress in several of the approved tasks. First, we have generated stable tissue culture cell lines which express human HER-2. These cell lines are an important component of our plan to test for specificity of infection through the single chain antibodies (sFv) to HER-2, and will be necessary for tittering by plaque assay. Secondly, our previous hybrids composed of the anti-Her-2 sFv linked to fragments of the SV5 HN glycoprotein were found to be very inefficiently transported to the cell surface. These proteins would be poor candidates to replace the normal attachment protein HN, since it is very likely that no infectious virus would be generated. As part of the original task 1, alternative approaches were employed to determine requirements for incorporation of sFv into virions. As such, genes were constructed such that the sFv was attached to the end of full length HN. Alternatively, a gene was made such that the sFv was attached to SH, a second SV5 membrane protein. Immunofluorescence demonstrated efficient cell surface expression of both Hnfull and SH-sFv. Infectious clones harboring these novel attachment proteins have been generated and will be used to generate infectious virus.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2002
- Accession Number
- ADA404653
Entities
People
- Griffith D. Parks
Organizations
- Wake Forest University