Development of a Novel Vaccine with Fusions of Dendritic and Ovarian Cancer Cells from Patients
Abstract
Ovarian carcinoma cells from patient were fused to autologous and allogeneic dendritic cells (DC). The fusion cells co-expressed tumor-associated antigens derived from ovarian carcinoma cells and DC-derived co-stimulatory molecules. Fusion cells retained the functional potency of DC and stimulated autologous T cell proliferation in vitro. These T cells derived from patients with metastatic ovarian cancer induced MHC class 1-restricted lysis of autologous ovarian tumor cells, but not monocytes allogeneic ovarian carcinoma cells or MUC1 positive tumor cell lines. These findings indicate that fusions of human ovarian cancer cells with autologous or allogeneic DC induce specific antitumor immune response against autologous ovarian cancer cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2001
- Accession Number
- ADA404671
Entities
People
- Jianlin Gong
Organizations
- Dana–Farber Cancer Institute