Y Chromosome Aberrations in Prostate Cancer
Abstract
We hypothesize that chromosomal loss of the Y chromosome in prostate cancer is a specific tumorigenic event. Our studies are to prove whether loss of a specific region of the Y chromosome is indicative of a tumor suppressor gene. We transferred the Y chromosome by microcell mediated chromosome transfer into PC-3 cells to determine if it suppressed tumor growth. Of the %5 mice injected with several independent clones only one produced a tumor in nude mice in contrast to control PC-3 cells. Our second approach is to determine the frequency of loss of the Y chromosome in prostate tumors. PCR analysis of Y specific loci on prostate cancer samples and FISH is used to identify the region of loss. Smaller chromosomal fragments are being used in the transfer experiments to define the region. Data from these experiments suggest that a tumor suppressor gene is located near the selectable marker on the short arm. The second approach is to develop a genomic microarray specific for the Y chromosome. BAC clones have been isolated and arrayed. Successful identification of a gene on the Y chromosome will provide a marker that may aid in the diagnosis and prediction of prognosis of prostate cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2001
- Accession Number
- ADA404706
Entities
People
- Susan L. Naylor
Organizations
- University of Texas Health Science Center at San Antonio