New Approaches for Early Detection of Breast Tumor Invasion or Progression
Abstract
To assess interactions between epithelial (EP) and myoepithelial (ME) cells in association with breast tumor progression and invasion, consecutive sections were immunostained with antibodies to estrogen receptor (ER), smooth muscle actin (SMA), and other biomolecules, and then microdissected for LOR and microsatellite instability (MI) assessments. Focal losses of ER expression in EP cells and disruptions of subjacent ME layers are correlated events in ER (+) tumors, whereas focal alterations of p27 expression in EP cells and disruptions of subjacent ME layers are correlated events in ER (-) tumors, suggesting that progression or invasion of these tumors may be regulated by different mechanisms. Cells in ducts with disrupted ME cell layers showed a substantially higher proliferation rate, and a vast majority of ER (-) cells overlying disrupted ME cell layers showed a marked higher frequency and different pattern of LOR and MI, compared to adjacent ER (+) cells within the same duct. These findings are in an agreement with our hypothesis that ER (-) cells overlying disrupted ME cell layer represent a more aggressive clone, and that simultaneous assessments of the immunohistochemical and genetic profiles of EP and ME cells could be a more sensitive approach for early detection of breast tumor progression or invasion.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2002
- Accession Number
- ADA404732
Entities
People
- Yan-gao Man