Demonstration that a mRNA Binding Protein is Responsible for GADD45 mRNA Destabilization
Abstract
We are studying the post-transcriptional control of expression of the p53-inducible antiproliferative gene known as Growth Arrest and DNA Damage induced gene 45 (GADD45). Using human breast carcinoma cell lines, we have demonstrated that the half-life of GADD45 mRNA is very responsive to ambient glutamine (GLN) availability. We have cloned the GADD45 cDNA and made several constructs of this cDNA that represent a first step toward making the constructs needed to test the region that is responsible for mRNA destabilization. We have also been developing the transfection techniques needed to conduct the mRNA stability studies. Unfortunately, the model system, TSE cells, has proven to be relatively difficult to transfect at a reasonable frequency. We are working to improve this frequency by using alternative transfection methods. Due to a shortage of technical help, the aims have not been completed in the first year. We have applied for a one-year no-cost extension to the award period.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2002
- Accession Number
- ADA404798
Entities
People
- Steve F. Abcouwer
Organizations
- University of New Mexico