A New APC-Like Gene Involved in Regulation of B-Catenin/LEF
Abstract
A second adenomatous polyposis coli (APC)-like gene, APC2/APCL was recently described and localized to chromosome 19. We have fine mapped APC2 to a small region of chromosome 19p13.3 containing markers D19S883 and WI-I 9632, a region commonly lost in a variety of cancers, particularly ovarian cancer. Interphase FISH analysis revealed an APC2 allelic imbalance in 19 of 20 ovarian cancers screened and indicates that APC2 could be a potential tumor suppressor gene in ovarian cancer. When over-expressed in SKOV3 ovarian cancer cells, which express low levels of APC2, exogenous APC2 localized to the Golgi apparatus, actin-containing structures, and occasionally to microtubules. Antibodies against the N-terminus of human APC2 show that endogenous APC2 is diffusely distributed in the cytoplasm and co-localizes with both the Golgi apparatus and actin filaments. APC2 remained associated with actin filaments following treatment with the actin-disrupting agent, cytochalasin D. These results suggest that APC2 is involved in actin associated events and could influence cell motility or adhesion through interaction with actin filaments as well as functioning independently or in cooperation with APC to down-regulate beta-catenin signaling.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2001
- Accession Number
- ADA404820
Entities
People
- Christy R. Jarrett
Organizations
- Georgetown University