Recombinant Antibodies for Biological Warfare Detection

Abstract

Recombinant antibodies to biological warfare threat agents including F. tularensis, Y. pestis, Brucella spp., V. cholerae O1 and O139, ricin, staphylococcal enterotoxins B and C, botulinum toxins A, B, and E and cholera toxin have been developed through the use of phage display technology. Both recombinant scFvs and Fabs have been produced. Substitutions of currently available monoclonal antibodies with these recombinant antibodies in immunological based detection systems have been successful. The recombinant antibodies exhibited equal sensitivity and equal or lower background across a number of platforms including ELISA assays, ECL based platforms and hand-held immunochromatographic assays. In addition, incorporation of the recombinant antibodies into current detection systems provides a stable genetic source for maintaining critical immunological reagents. The use of recombinant antibodies has allowed for improved detection and identification of biological warfare agents.

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Document Details

Document Type
Technical Report
Publication Date
Aug 13, 2002
Accession Number
ADA404874

Entities

People

  • Amanda Dim-schultz
  • Joan S. Gebhardt

Organizations

  • Naval Medical Research Center

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Biological Factors
  • Biological Products
  • Biological Warfare
  • Biological Warfare Agents
  • Biomedical Research
  • Chemical Compounds
  • Culture Techniques
  • Detection
  • Identification
  • Military Operations
  • Platforms
  • Public Health
  • Recombinant Dna
  • Sensitivity
  • Warfare

Fields of Study

  • Biology

Readers

  • Critical Infrastructure Protection in CBRN and WMD Threats.
  • Microbial Pathology
  • Molecular and Cellular Biochemistry

Technology Areas

  • Biotechnology